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  • 试剂盒 > 酶联免疫试剂盒 > 人载脂蛋白A1(Apo-A1)ELISA kit
      • ELISA Kit
      • 人载脂蛋白A1(Apo-A1)ELISA kit
      • This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.[provided by RefSeq]
      • UniProtP02647
      • BIOWWWAPOA1
      • HGNC600
      • RGD2130
      • MGI88049
      • OMIM107680
      • CUSABIOCSB-E08103h
    • 产品类型:
      ELISA Kit
    • 产品名称:

      黑金团队快乐8官方网址:人载脂蛋白A1(Apo-A1)ELISA kit

    • 英文名称:

      Human apolipoprotein A1 (Apo-A1) ELISA Kit

    • 货号:
      CSB-E08103h
    • 别名:
      MGC117399, OTTHUMP00000069346|OTTHUMP00000069347|OTTHUMP00000069348
    • 规格:
      96T
    • 中文价格:
      3600
    • 种属:
      Homo sapiens (Human)
    • 待测物名称:
      apolipoprotein A-I
    • 缩写:
      APOA1
    • 蛋白功能1:
      Apolipoprotein
    • 蛋白功能2:
      Lipogenesis and lipometabolism
    • 蛋白功能3:
      Cholesterol metabolism
    • 检测范围:
      1.4 ng/mL-1000 ng/mL
    • 灵敏度:
      0.7 ng/mL
    • 反应时间:
      1-5h
    • 所需样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 下载:
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
      ng/ml OD1 OD2 Average Corrected  
      1000 1.879 1.903 1.891 1.728  
      500 1.592 1.612 1.602 1.439  
      250 1.304 1.311 1.308 1.145  
      125 0.955 0.968 0.962 0.799  
      62.5 0.743 0.757 0.750 0.587  
      31.2 0.562 0.572 0.567 0.404  
      15.6 0.374 0.376 0.375 0.212  
      0 0.161 0.165 0.163    
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%      
      Three samples of known concentration were tested twenty times on one plate to assess.  
      Inter-assay Precision (Precision between assays): CV%<10%      
      Three samples of known concentration were tested in twenty assays to assess.    
                   
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
        Sample Serum(n=4)  
      1:2000 Average % 88  
      Range % 84-92  
      1:4000 Average % 96  
      Range % 92-102  
      1:8000 Average % 95  
      Range % 89-100  
      1:16000 Average % 92  
      Range % 89-96  
    • 回收率:
      The recovery of human Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
      Sample Type Average % Recovery Range  
      Serum (n=5) 94 89-98  
      EDTA plasma (n=4) 96 91-101  
                   
                   
    • 定性/定量:
      quantitative
    • 研究领域:
      Cardiovascular
    • 引用文献:
      1. Discovery of Apo-A1 as a potential bladder cancer biomarker by urine proteomics and analysis , Li C et al,Biochem Biophys Res Commun,2014 Mar 21. pii: S0006-291X(14)00496-3.
      2. Identification of Differentially Expressed Proteins in the Serum of Colorectal Cancer Patients Using 2D-DIGE Proteomics Analysis , Lay Cheng Lim,.et al,Pathology & Oncology Research,2016,22(1):169-177.
      3. A study of serum Apo A-1 and Apo B-100 levels in women with preeclampsia , Timur H.et al,Pregnancy Hypertens.,6(2):121-5
      4. The barberry juice effects on metabolic factors and oxidative stress in patients with type 2 diabetes: A randomized clinical trial , FatemehLazavi.et al,Complementary Therapies in Clinical Practice,May