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  • 试剂盒 > 酶联免疫试剂盒 > 小鼠叶酸(FA)ELISA Kit
      • ELISA Kit
      • 小鼠叶酸(FA)ELISA Kit
    • 产品类型:
      ELISA Kit
    • 产品名称:

      北京快乐八开奖结果:小鼠叶酸(FA)ELISA Kit

    • 英文名称:

      Mouse Folic acid,FA ELISA Kit

    • 货号:
      CSB-E08758m
    • 别名:
      N/A
    • 规格:
      96T
    • 中文价格:
      3800
    • 种属:
      Mus musculus (Mouse)
    • 其他种属:
    • 待测物名称:
      Folic acid,FA
    • 缩写:
      FA
    • 蛋白功能1:
      Metabolism
    • 检测范围:
      0.195 ng/mL-50 ng/mL
    • 灵敏度:
      0.195 ng/mL
    • 反应时间:
      1-5h
    • 所需样本体积:
      50-100ul
    • 检测波长:
      450 nm
    • 下载:
    • 标准曲线:
      These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
       
      ng/ml OD1 OD2 Average      
      50 0.321 0.332 0.327      
      12.5 0.512 0.537 0.525      
      3.12 0.826 0.851 0.839      
      0.78 1.175 1.153 1.164      
      0.195 1.655 1.632 1.644      
      0 1.985 1.948 1.967      
    • 精密度:
      Intra-assay Precision (Precision within an assay): CV%<8%        
      Three samples of known concentration were tested twenty times on one plate to assess.    
      Inter-assay Precision (Precision between assays): CV%<10%        
      Three samples of known concentration were tested in twenty assays to assess.      
                     
    • 线性度:
      To assess the linearity of the assay, samples were spiked with high concentrations of mouse FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
        Sample Serum(n=4)    
      1:1 Average % 97    
      Range % 90-105    
      1:2 Average % 89    
      Range % 83-96    
      1:4 Average % 92    
      Range % 84-99    
      1:8 Average % 91    
      Range % 85-99    
    • 回收率:
      The recovery of mouse FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
       
      Sample Type Average % Recovery Range    
      Serum (n=5) 95 87-100    
      EDTA plasma (n=4) 95 89-99    
                     
                     
    • 定性/定量:
      quantitative
    • 研究领域:
      Metabolism
    • 引用文献:
      1. Folic acid prevents cardiac dysfunction and reduces myocardial fibrosis in a mouse model of high-fat diet-induced obesity , Li W.et al,Nutr Metab (Lond),Nov 2